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Chemistry and Molecular Biology Postdoctoral Associates
University of Central Florida in Orlando, Florida
 
 
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Date Posted 03/06/2019
Category
Postdoctoral-Biology
Salary $24 -  USD Per Hour
Employment Type Fulltime
Application Deadline Open until filled
 
 
 
 
 

Position Number

20605

Class Title

Chemistry and Molecular Biology Postdoctoral Associates

Administrative Title

No Administrative Function

Job Description

Project overview:
The goal is to develop and optimize a new therapy for patients with pancreatic ductal adenocarcinoma and the project will identify and develop new drug candidates for the treatment of pancreatic cancers. The UCF team will investigate new polyamine transport inhibitors as well as the regulation of genes associated with polyamine transport in human pancreatic cancers.

The postdoc will:
a) use CRISPR technology to generate stable cell lines with varying levels (knockout vs overexpression) of two polyamine transport genes (ATP13A3 and SLC12A8); 
b) use qrt-PCR and Western blots to evaluate the degree of full length and truncated mRNA and protein levels of two polyamine transport proteins (ATP13a3 and SLC12A8);
c) determine the polyamine uptake kinetics (Vmax and Km values) of 3H-Spermidine in the new cell lines in the presence and absence of polyamine stimuli;
d) determine the performance of the new transport inhibitors using two orthogonal polyamine transport assays (DFMO/Ant44) in the new cell lines;
e) perform confocal microscopy studies to look at cell localization changes of key polyamine transport proteins (ATP13A3 and SLC12A8) in each cell line in the presence and absence of exogenous polyamines;
f) train graduate students in molecular biology techniques;
g) generate semester reports for inclusion in the grant yearly reports and;
h) write manuscripts and grant proposals related to the project.

Position Minimum Qualifications

For the chemistry position, a Ph.D. in Organic Chemistry, Biochemistry or equivalent from an accredited institution.

For the molecular biology position, a Ph.D. in Molecular Biology or Biomedical Sciences or equivalent from an accredited institution.

Preferences

The preferred candidate for the chemistry position will have experience with organic synthesis and conducting biochemical assays, as well as expertise in TLC, HPLC, chromatography, NMR, LC-MS, optical rotation measurements, column chromatography, confocal microscopy, drug design and molecular modeling experience with PyMol or equivalent software.

The preferred candidate for the molecular biology position will have experience with cell culture, protein isolation, Western blot, RNAisolation, qrt PCR, DNA sequencing, CRISPR transfections, ELISA assays, and confocal microscopy techniques.

Special Conditions

Applicants for this position will also be considered for position number 20604.

This is a repost position. Previous applicants are still being considered and do not need to reapply.

Equal Employment Opportunity Employer

As an equal opportunity/affirmative action employer, UCF encourages all qualified applicants to apply, including women, veterans, individuals with disabilities, and members of traditionally underrepresented populations. UCF's Equal Opportunity Statement can be viewed at: http://www.oie.ucf.edu/documents/PresidentsStatement.pdf. As a Florida public university, UCF makes all application materials and selection procedures available to the public upon request.

Additional Application Materials Required

UCF requires all applications and supporting documents to be submitted
electronically through the Human Resources website, www.jobswithucf.com.
In addition to the online application, interested applicants must submit the
following documents at the time of application:
(1) a signed cover letter;
(2) a complete curriculum vitae;
(3) a list of at least three (3) professional references which includes addresses, phone numbers, and email addresses.

NOTE: Please have all documents ready when applying so they can be attached at that time. Once the online submission process is finalized, the system does not allow applicants to submit additional documents at a later date.

Questions regarding this search may be directed to Floyd Sturdifen at 407-266-1226.

FTE

1.0-Full-Time

Requisition Number

701289

Job Open Date

05/25/2018

Job Close Date

09/13/2018

Open until filled

No

Division

Academic Affairs

College/Area

College of Medicine (COM)

Department

College of Medicine

Annual Salary

$24 per hour

Type of Appointment

Postdoctoral

Job Category

Postdoctoral

Work Location

Orlando (Main)

Quick Link

http://www.jobswithucf.com/postings/53436

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